Nnsolid phase dna synthesis pdf files

Solid phase peptide synthesis, strategies and resins. Benefits compared with normal synthesis in a liquid state include. Solid phase versus solution phase synthesis of heterocyclic macrocycles article pdf available in molecules 181. Solid phase synthesis of 5 triantennary nacetylgalactosamine conjugated antisense oligonucleotides using phosphoramidite chemistry. To increase their synthetic diversity, we report a mild methodology based on 4methyltrityl mtt solid phase peptide synthesis spps, whose building blocks are more accessible than the standard fmoc and boc spps ones.

A simple method for preparation and characterization of aristolochic aciddna adducts was developed. The strategy involves exploding an immobilized aldehyde into a plethora of different functional groups under dnacompatible conditions. Isocyanide multicomponent reactions on solidphasecoupled. This synthesis method is difficult to control resulting in agglomeration of the enm and is thus rarely used and will not be discussed further 32. Although dna exhibits limited solubility in nonaqueous solvents, its immobilization on a solid phase can be exploited to access such environments. Solidphase synthesis of dnaencoded libraries via an. Solid phase peptide synthesis part i applications of bocbzl strategy g bor mez research group of peptide chemistry hungarian academy of sciences powerpoint ppt presentation. The ugi, ugiazide, and groebkeblackburnbienayme reactions are welltolerated by dna on the solid phase and show a broad scope. Solidphase synthesis has a number of advantages over solution synthesis. However, an oxadiazoleforming variant of the ugi reaction caused dna depurination, requiring a more stable hexathymidine dna for encoded library synthesis. Dnabinding polyamides are synthetic oligomers of pyrroleimidazole units with high specificity and affinity for doublestranded dna. In chemistry, solid phase synthesis is a method in which molecules are covalently bound on a solid support material and synthesised stepbystep in a single reaction vessel utilising selective protecting group chemistry. Solid phase synthesis an overview sciencedirect topics. High efficiency and throughput increased simplicity and speed the reaction can be driven to completion and high yields through the use of excess reagent.

With a solidphase chemistry approach, a large existing body of known chemical transformations becomes accessible to dnaencoded synthesis. Aristolochic acid aa is a known nephrotoxin and potential carcinogen, which can form covalent dna adducts after metabolic. Solidphase synthesis is carried out on a solid support held between filters, in columns that enable all reagents and solvents to pass through freely. In this method, building blocks are protected at all react. Pdf solid phase versus solution phase synthesis of. Spps is more efficient, faster and the purification is easier omethod often used in chemistry and biochemstry. The solid vapor synthesis method is technically classified as a vapor aerosol phase synthesis and involves the reaction between solid and gas precursors. We report chemistry suitable for the solidphase synthesis of dnaencoded libraries with an unusually high level of structural diversity.